Paper based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition. Paper substrates have been widely used to construct point of care lateral flow immunoassay diagnostic devices like the home pregnancy test. In 2008, the group introduced the first 3d microfluidic paperbased analytical devicesalso referred to as pads. We will also identify challenges to commercialization. Paperbased analytical devices for clinical diagnosis.
Selfpowered integrated microfluidic pointofcare low. In fact, they can not only be used for biochemical analysis but also for medical and forensic diagnostics. Successful device integration can be achieved by a twopart approach composed of a systemlevel view of the whole device and individual module development 84. Integrating microfluidics, optical sensing modality, and smartphones can provide a powerful platform to develop affordable and easily. Threedimensional microfluidic paperbased analytical devices 3d. Odevelopment of pointofcare diagnostic assays using. Paperbased pointofcare testing in disease diagnostics. We define paper broadly as a variety of porous materials, although the emphasis often is on traditional cellulosic forms. Paperbased microfluidic devices have a great potential to be a good alternative to traditional microfluidic systems for pointofcare diagnostic applications. Early microfluidicbased pointofcare diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. Lowe, journallab on a chip, year20, volume 12, pages 221051. The first system, using digital microfluidics, is reusable and allows for precise fluid control. Microfluidic paperbased analytical devices micropads began as a simple idea with an ambitious goal.
There was a dramatic rise in the use of paper in pointofcare diagnostics after two reports in 2007 demonstrated that. A chemically patterned microfluidic paperbased analytical. Whitesides5 in developed nations, monitoring for dr uginduced liver injury through serial. Paperbased point of care diagnostics posner research group. Poc devices have been found to have many advantages like a rapid and precise response, portability, low cost, and non. Since their introduction in 2007, paperbased microfluidic devices micropads have been explored extensively as platforms for pointofcare diagnostic tests and as tools for basic research and. Paperbased systems for pointofcare biosensing sherine. Special issue paperbased microfluidic devices for point. But his teams aim is to enable microfluidics to perform a greatly expanded range of tests for everything from infectious diseases to drug. Mar 22, 2017 numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527.
The goal was to develop lowcost and portable paperbased diagnostic devices to improve healthcare in developing countries. Cheap ubiquitous lightweight can be disposed of by incineration, so doesnt produce biohazardous waste. Development of paper based point of care microfluidic diagnostic devices project number imura0628 monash main supervisor name, email id, phoneadrian neild, adrian. Pads paper is hydrophilic and porous, thus providing a natural platform for fabricating microfluidic channels. Paperbased 3d microfluidic device for multiple bioassays. A large part of the excitement behind microfluidics is in its potential for producing practical devices, but surprisingly few labonachip based technologies have been successfully introduced into the market. Pdf a historical perspective on paper microfluidic based. Fabrication of laser printed microfluidic paperbased.
Currently, the majority of poc diagnostics utilize paper substrates owing to affordability, disposability, and mass production capability. To tune fluid penetration in porous substrates such as paper in two and three dimensions, the pore structure, wettability and geometry of the microfluidic devices can be controlled. Pads can provide potentially relevant analysis for biomarker detection in many types of biological fluids, including tear fluids 28, 29, whole blood 30 32, serum 33, urine 34 and saliva. Portable, lowcost, and quantitative nucleic acid detection is desirable for pointofcare diagnostics. Apr 26, 2017 a chemically patterned microfluidic paperbased analytical device cpad is developed to create fluidic networks by forming hydrophobic barriers using chemical vapor deposition cvd of. Paper based microfluidic systems have emerged as one of the most favorable technologies used in many potential applications such as point of care diagnostics, flexible electronics, energy storage. Aug 22, 2017 this work describes the fabrication of self. The overall goal of this dissertation is to develop microfluidic diagnostic devices which are practical and reliable for global health. Moreover, each device can be used to measure more than one analyte. Pads and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrumentfree fast results. Paperbased microfluidic devices fill a growing niche for portable, cheap, and userfriendly medical diagnostic systems. The global microfluidics market size was evaluated at usd. Pads are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Development of point of care diagnostic assays using paper based microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the point of care has broad implications for the global management of healthcare.
Digital and paper microfluidic diagnostic devices design document 3 summary the project aims to design two complementary systems for performing biological assays and diagnostic tests. Paperbased devices are a portable, userfriendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. Recently, paperbased microfluidics has emerged as a multiplexable pointofcare platform which might transcend the capabilities of existing assays in resourcelimited settings. Since the development of the first commercially available devices in the 1990s, microfluidic technologies have been evolving to benefit an evergreater range of applications. Pads are expected to have profound implications, especially for in vitro diagnosis. Numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527. This platform opens new possible applications for paperbased pointof care poc diagnostic devices and enables the possibility of these devices to introduce time control functions without the. Microfluidic and paperbased devices for disease detection. Despite their significant scope, the complexity of fabrication. In addition, the basics of paperbased microfluidics including a short history of paperbased microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described.
Jun 23, 2017 paperbased analytical devices for clinical diagnosis. Fabrication of miniaturized paperbased microfluidic. Point of care poc diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. There was a dramatic rise in the use of paper in point of care diagnostics after two reports in 2007 demonstrated that. Pointofcare poc diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. Recently, paper has been functionalised as a substrate to construct microfluidic devices for use in rapid diagnostic tests. However, paper based microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental. Salivary diagnostics on paper microfluidic devices and.
Microfluidic systems as pointofcare diagnostic devices. Microfluidicbased pointofcare testing for global health tassaneewan laksanasopin. Multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home. While many devices focus on one of those four areas of detection, a few devices identifies fungi such as candida. We report an integrated microfluidic diagnostic device capable of onsite quantitative nucleic acid detection directly from the blood without separate sample preparation steps.
To listen to a podcast about this feature, please go to. Speds are inexpensive, lightweight, mechanically flexible, easy to use, and disposable by burning. Diseases such as periodontitis and diabetes mellitus can potentially be detected through these devices by the detection of important biomarkers. Pdf paperbased microfluidic pointofcare diagnostic. Apr 26, 2017 multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home. M bsa concentrations and 0250 mm glucose concentrations was used to investigate the relationship between two assays using the. Recently, paper based microfluidics has emerged as a multiplexable point of care platform which might transcend the capabilities of existing assays in resourcelimited settings. Rise in demand for point of care poc devices is expected to significantly drive the market. Microfluidics based devices need a fraction of the sample for data interpretation. Microfluidic paperbased analytical devices pads have provided a breakthrough in portable and lowcost point of care diagnostics. The expanding role of paper in pointofcare diagnostics. As a rapidly developing field, paperbased microfluidic devices.
Biomedical engineering a paperbased multiplexed transaminase test for lowcost, pointofcare liver function testing nira r. Newgeneration hospital centers are equipped with centralized diagnostic laboratories containing hightech analytical devices and clinical instruments, which are not feasible or affordable for many lessdeveloped countries. Paper based microfluidic point of care diagnostic devices received 6th february 20, accepted 18th march 20 doi. Paper substrates have been widely used to construct pointofcare lateral flow immunoassay diagnostic devices like the home pregnancy test. Pads are a new class of pointofcare diagnostic devices that are inexpensive, easy to use, and designed specifically for use in developing countries. The technology builds on the conventional lateral flow test which is capable of detecting many infections agents and chemical contaminants. Paper based microfluidic devices fill a growing niche for portable, cheap, and userfriendly medical diagnostic systems. Can be produced on a mass scale portable 6 what are microfluidic pointofcare diagnostic devices in the year 2004, the world.
This indicates that the smartphonebased 3d paperbased microfluidic device is a useful platform with great potential for application in pointofcare diagnostics. Pads are a new class of pointofcare diagnostic devices that are inexpensive. Recently, flexible polymer substrates have been investigated due to. However, paperbased microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental. The goal was to develop lowcost and portable paperbased diagnostic devices to. Paper based microfluidic point of care diagnostic devices. Paper based microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. Progress in the development of paperbased diagnostics for. Paperbased microfluidic pointofcare diagnostic devices.
The integration issues relevant to the creation of paper based diagnostic devices are analogous to the issues in conventional microfluidics based diagnostic device development. Beattie,2 sidhartha jain,2 farzad noubary,3 vicki l. The integration issues relevant to the creation of paperbased diagnostic devices are analogous to the issues in conventional microfluidicsbased diagnostic device development. Nevertheless, only singleanalyte approaches are typically. Rise in demand for pointofcare poc devices is expected to significantly drive the market. Apr 12, 2016 3d paper based microfluidics have the following advantages over 2d. Improving sample distribution homogeneity in three. A accommodates more assays on the same footprint of a device than a 2d device testing of different samples simultaneously within a compact space b moves fluid through the thickness of paper the zdirection and laterally the x,yplane. Microfluidic systems as point of care diagnostic devices.
Paperbased analytical devices pads as demonstrated by whitesidess group offer a promising solution for pointofcare diagnostics 2527. Development of pointofcare diagnostic assays using paperbased microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the pointofcare has broad implications for the global management of healthcare. Diagnostics for all, instrumentfree tests based on paper, capillarydriven microfluidics, with a colorimetric readout. Paperbased microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. This platform opens new possible applications for paper based point of care poc diagnostic devices and enables the possibility of these devices to introduce time control functions without the. Apr 26, 2019 in addition, the basics of paper based microfluidics including a short history of paper based microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described. A paperbased multiplexed transaminase test for lowcost. The main advantage of this is that it is largely a passively. The future of microfluidic pointofcare diagnostic devices. Paperbased diagnostic microfluidic devices medgadget. Jun 28, 2010 early microfluidic based point of care diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. From microfluidic paperbased analytical devices to paper. Pointofcare microfluidic devices for pathogen detection.
Making paper diagnostic tests, made while he was working. Microfluidicbased pointofcare testing for global health. Integrated paperbased microfluidic devices for pointof. An automated smartphonebased diagnostic assay for point. Biological detection biological activities can be recognized by different signal transduction pathways performing on the. Paperbased microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition.
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